Plasmid
ACT1p-scFv

Part:BBa_K4203000:Design

Designed by: Yixuan Wang; Zongjun Lee   Group: iGEM22_BJWZ-China   (2022-10-12)


After codon optimization, those part is suitable for the fusion protein ACT1P-scFv-GFP-actin express


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3846
    Illegal XbaI site found at 4647
    Illegal XbaI site found at 8717
    Illegal PstI site found at 395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5546
    Illegal PstI site found at 395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4185
    Illegal BglII site found at 4764
    Illegal BamHI site found at 5327
    Illegal BamHI site found at 5454
    Illegal XhoI site found at 5524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3846
    Illegal XbaI site found at 4647
    Illegal XbaI site found at 8717
    Illegal PstI site found at 395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3846
    Illegal XbaI site found at 4647
    Illegal XbaI site found at 8717
    Illegal PstI site found at 395
    Illegal NgoMIV site found at 1676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1085
    Illegal BsaI.rc site found at 6887
    Illegal SapI site found at 5804
    Illegal SapI.rc site found at 932


Design Notes

Pay attention to the selection of Linker length and species, otherwise the efficiency of protein expression will be seriously affected


Source

Saccharomyces cerevisiae S288C actin (ACT1), partial mRNA(NM_001179927.1) The sequences of scFv and superfolder GFP come from pHR-scFv-GCN4-sfGFP-GB1

References