Plasmid
ACT1p-scFv
Part:BBa_K4203000:Design
Designed by: Yixuan Wang; Zongjun Lee Group: iGEM22_BJWZ-China (2022-10-12)
After codon optimization, those part is suitable for the fusion protein ACT1P-scFv-GFP-actin express
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3846
Illegal XbaI site found at 4647
Illegal XbaI site found at 8717
Illegal PstI site found at 395 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5546
Illegal PstI site found at 395 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4185
Illegal BglII site found at 4764
Illegal BamHI site found at 5327
Illegal BamHI site found at 5454
Illegal XhoI site found at 5524 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3846
Illegal XbaI site found at 4647
Illegal XbaI site found at 8717
Illegal PstI site found at 395 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3846
Illegal XbaI site found at 4647
Illegal XbaI site found at 8717
Illegal PstI site found at 395
Illegal NgoMIV site found at 1676 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1085
Illegal BsaI.rc site found at 6887
Illegal SapI site found at 5804
Illegal SapI.rc site found at 932
Design Notes
Pay attention to the selection of Linker length and species, otherwise the efficiency of protein expression will be seriously affected
Source
Saccharomyces cerevisiae S288C actin (ACT1), partial mRNA(NM_001179927.1) The sequences of scFv and superfolder GFP come from pHR-scFv-GCN4-sfGFP-GB1